A Coding Guide to Building a Complete Pipeline for Single-Cell Sequencing Analysis Using Scanpy for Clustering and Cell Type Annotation
In this tutorial, we build a complete pipeline for single-cell RNA sequencing using Scanpy. We start by installing the necessary libraries and loading the PBMC 3k dataset, then perform quality control, sorting, and normalization to prepare the data for upstream analysis. We then identified the most mutated genes, performed dimensionality reduction PCA, and constructed a … Read more